Journal: International Journal of Molecular Medicine

Article Title: Calycosin attenuates mitochondrial damage and pyroptosis in heart failure via the Nrf2/ROS/TXNIP pathway

doi: 10.3892/ijmm.2025.5653

Figure Lengend Snippet: CA improves pyroptosis in HF through the Nrf2/ROS/TXNIP pathway. (A) Representative Nrf2 immunohistochemistry images of each group and their quantitative analysis (n=3). (B) Representative images of the expression levels of Nrf2 and TXNIP in myocardial tissue of Wistar rats assessed by western blotting. (C) Semi-quantitative analysis of Nrf2 and TXNIP expressions (n=3). (D) Representative images of the expression levels of NLRP3, ASC, pro- and cleaved-caspase-1, GSDMD, GSDMD-NT in myocardial tissue of Wistar rats by western blotting. (E) Semi-quantitative analysis of NLRP3, ASC, pro- and cleaved-caspase-1, GSDMD, GSDMD-NT expressions (n=3). (F) Representative images of the expression levels of IL-18 and IL-1β in myocardial tissue of Wistar rats by western blotting. (G) Semi-quantitative analysis of IL-18 and IL-1β expressions (n=3). Data are presented as the mean ± SD, * P<0.05, ** P<0.01, *** P<0.001. CA, calycosin; HF, heart failure; Nrf2, nuclear factor erythroid 2-related factor; ROS, reactive oxygen species; TXNIP, thioredoxin-interacting protein; CA-L, calycosin low-dose; CA-H, calycosin high-dose; ASC, apoptosis-associated speck-like protein containing a CARD; GSDMD, gasdermin D; GSDMD-NT, GSDMD N-terminal fragments.

Article Snippet: Antibody information was as follows: Nrf2 (cat. no. 80593-1-RR), apoptosis-associated speck-like protein containing a CARD (ASC; cat. no. 67494-1-Ig), IL-1β (cat. no. 29530-1-AP), cytochrome c (cat. no. 66264-1-Ig) and β-actin (cat. no. 66009-1-Ig) antibodies were purchased from Proteintech Group, Inc.; TXNIP (cat. no. A9342), NLRP3 (cat. no. A5652), IL-18 (cat. no. A23076) and GSDMD (full length and N terminal; cat. no. A24476 ) antibodies were purchased from ABclonal Biotech Co., Ltd.; Caspase-1 (pro- and cleaved-Caspase-1) (cat. no. ab286125), COX IV (cat. no. ab16056), Goat Anti-rabbit IgG HRP (cat. no. ab150077) and Goat Anti-Mouse IgG HRP (cat. no. ab205719) antibodies were purchased from Abcam.

Techniques: Immunohistochemistry, Expressing, Western Blot

Journal: International Journal of Molecular Medicine

Article Title: Calycosin attenuates mitochondrial damage and pyroptosis in heart failure via the Nrf2/ROS/TXNIP pathway

doi: 10.3892/ijmm.2025.5653

Figure Lengend Snippet: CA inhibits pyroptosis via the Nrf2/reactive oxygen species/TXNIP pathway (n=3). (A) Representative images of the expression levels and semi-quantitative analysis of Nrf2 and TXNIP in cells by western blotting (n=3). (B) and semi-quantitative analysis and semi-quantitative analysis of IL-18 and IL-1β in cells by western blotting and (n=3). (C) and semi-quantitative analysis and semi-quantitative analysis of levels of NLRP3, ASC, pro- and cleaved-caspase-1, GSDMD, GSDMD-NT in cells by western blotting (n=3). Data are presented as the mean ± SD, * P<0.05, ** P<0.01, *** P<0.001. CA, calycosin; Nrf2, nuclear factor erythroid 2-related factor; TXNIP, thioredoxin-interacting protein; H/R, hypoxia-reperfusion, ASC, apoptosis-associated speck-like protein containing a CARD; GSDMD, gasdermin D; GSDMD-NT, GSDMD N-terminal fragments; NLRP3, NLR family pyrin domain-containing protein 3.

Article Snippet: Antibody information was as follows: Nrf2 (cat. no. 80593-1-RR), apoptosis-associated speck-like protein containing a CARD (ASC; cat. no. 67494-1-Ig), IL-1β (cat. no. 29530-1-AP), cytochrome c (cat. no. 66264-1-Ig) and β-actin (cat. no. 66009-1-Ig) antibodies were purchased from Proteintech Group, Inc.; TXNIP (cat. no. A9342), NLRP3 (cat. no. A5652), IL-18 (cat. no. A23076) and GSDMD (full length and N terminal; cat. no. A24476 ) antibodies were purchased from ABclonal Biotech Co., Ltd.; Caspase-1 (pro- and cleaved-Caspase-1) (cat. no. ab286125), COX IV (cat. no. ab16056), Goat Anti-rabbit IgG HRP (cat. no. ab150077) and Goat Anti-Mouse IgG HRP (cat. no. ab205719) antibodies were purchased from Abcam.

Techniques: Expressing, Western Blot

Journal: International Journal of Molecular Medicine

Article Title: Calycosin attenuates mitochondrial damage and pyroptosis in heart failure via the Nrf2/ROS/TXNIP pathway

doi: 10.3892/ijmm.2025.5653

Figure Lengend Snippet: Silencing Nrf2 inhibits the therapeutic effects of CA. (A) Transfection efficiency of si-Nrf2. (B) Detection of intracellular ROS levels in each group (n=3). (C) Representative images and semi-quantitative analysis of Nrf2 and TXNIP in cells by western blotting (n=3). (D) Representative images and semi-quantitative analysis of NLRP3, ASC, pro- and cleaved-caspase-1, GSDMD, GSDMD-NT in cells by western blotting (n=3). (E) Representative images and semi-quantitative analysis levels of IL-18 and IL-1β in cells by western blotting (n=3). Data are presented as the mean ± SD, * P<0.05, ** P<0.01, *** P<0.001, ns, no significance. si, small interfering; CA, calycosin; Nrf2, nuclear factor erythroid 2-related factor; ROS, reactive oxygen species; TXNIP, thioredoxin-interacting protein; ASC, apoptosis-associated speck-like protein containing a CARD; NLRP3, NLR family pyrin domain-containing protein 3; GSDMD, gasdermin D; GSDMD-NT, GSDMD N-terminal fragments; H/R, hypoxia-reperfusion; NC, negative control.

Article Snippet: Antibody information was as follows: Nrf2 (cat. no. 80593-1-RR), apoptosis-associated speck-like protein containing a CARD (ASC; cat. no. 67494-1-Ig), IL-1β (cat. no. 29530-1-AP), cytochrome c (cat. no. 66264-1-Ig) and β-actin (cat. no. 66009-1-Ig) antibodies were purchased from Proteintech Group, Inc.; TXNIP (cat. no. A9342), NLRP3 (cat. no. A5652), IL-18 (cat. no. A23076) and GSDMD (full length and N terminal; cat. no. A24476 ) antibodies were purchased from ABclonal Biotech Co., Ltd.; Caspase-1 (pro- and cleaved-Caspase-1) (cat. no. ab286125), COX IV (cat. no. ab16056), Goat Anti-rabbit IgG HRP (cat. no. ab150077) and Goat Anti-Mouse IgG HRP (cat. no. ab205719) antibodies were purchased from Abcam.

Techniques: Transfection, Western Blot, Negative Control